Zebrafish Rab10 Antibody

Ras-related protein Rab-10 is a protein that in humans is encoded by the RAB10 gene. RAB10 belongs to the RAS superfamily of small GTPases. And RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking. The RAB10 gene is mapped to chromosome 2p23.1-p22.3 by radiation hybrid analysis. It was found that the purified recombinant GAP domain of human AS160 showed GAP activity with RAB2A, RAB8A, RAB10, and RAB14, but not with 14 other RABs. Immunoblot analysis showed that these RABs associated with Glut4-positive vesicles in mouse adipocytes. Thereby, it concluded that AK160 functions as a RAB GAP and that RABs may participate in GLUT4 translocation.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL

UniProt

Q6DGV5

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Rab10 recombinant protein (amino acids A153-C200) was used as the immunogen for the Zebrafish Rab10 antibody.

Clonality

Polyclonal

Isotype

Applications

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Rab10 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Rab10 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Rab10 antibody should be determined by the researcher.

Image Legend

Western blot analysis of Rab10 protein using Zebrafish Rab10 antibody and 1) whole female zebrafish and 2) 1) whole male zebrafish tissue lysate. Predicted molecular weight ~22 kDa.