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Zebrafish Ube2n Antibody / Isoforms a & b

Predicted to enable ubiquitin conjugating enzyme activity. Acts upstream of or within DNA damage response and protein polyubiquitination. Predicted to be active in nucleus. Orthologous to several human genes including UBE2N (ubiquitin conjugating enzyme E2 N) .

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q803J2, Q7T3F3

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish recombinant protein (amino acids Q13-I152) was used as the immunogen for the Zebrafish Ube2n antibody. This antibody will detect the a and b isoforms.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Ube2n antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Ube2n antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Product Datasheet

http://www.nsjbio.com/tds-pdf/zebrafish-ube2n-antibody-rz1020

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Ube2n antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE Zebrafish liver tissue with Ube2n antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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