Zebrafish Gls Antibody / Glutaminase / Isoforms a & b

This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

A0A8M9PRT8

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Glsa/b recombinant protein (amino acids K53-S312) was used as the immunogen for the Zebrafish Gls antibody. This antibody will detect the a and b isoforms.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Gls antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Gls antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Gls antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE zebrafish heart tissue with Zebrafish Gls antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.