Zebrafish Ckm Antibody / Creatine kinase M / Isoforms a & b

Creatine kinase, muscle also known as CKM is a creatine kinase that in humans is encoded by the CKM gene. This gene is mapped to 19q13.32. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

A2BHA3

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Ckma/b recombinant protein (amino acids M1-K196) was used as the immunogen for the Zebrafish Ckm antibody. This antibody will detect the a and b isoforms.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Ckm antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Ckm antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Ckm antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE zebrafish spleen tissue with Zebrafish Ckm antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.