Zebrafish Eif4a1 Antibody / Isoforms a & b / EIF4A2

Eukaryotic initiation factor 4A-I is a protein that in humans is encoded by the EIF4A1 gene. It is mapped to 17p13.1. EIF4A1 has been shown to interact with EIF4E and eukaryotic translation initiation factor 4 gamma.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q802C9

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Eif4a1a/b/2 recombinant protein (amino acids D32-I406) was used as the immunogen for the Zebrafish Eif4a1 antibody. This antibody will detect the a and b isoforms of Eif4a1 as well as Eif4a2.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Eif4a1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Eif4a1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Eif4a1 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE zebrafish brain tissue with Zebrafish Eif4a1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.