Zebrafish Jab1 Antibody / Jun activation domain-binding protein 1 / Cops5

COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis), also known as COPS5 or JAB1, is a gene conserved from humans to Saccharomyces cerevisiae. It is a member of the MOV34 family. COPS5 is mapped to 8q13.1. The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. COPS5 can interact with the cytoplasmic domain of the beta-2 subunit of the alpha-L/beta-2 integrin LFA1, and it is the only protein demonstrated to interact with MIF. COPS5, VHL, and TRC8 proteins appear to be linked both physically and functionally, and all 3 may participate in the development of kidney cancer. In addition to that, COPS5 is an essential cofactor for the apoptotic function of E2F1.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q6PC30

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Jab1/COPS5 recombinant protein (amino acids I6-S332) was used as the immunogen for the Zebrafish Jab1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Jab1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Jab1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Jab1 antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

IHC staining of FFPE zebrafish pancreas tissue with Zebrafish Jab1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.