Zebrafish Dhx9 Antibody / RNA Helicase

ATP-dependent RNA helicase A (RHA; also known as DHX9, LKP, and NDHI) is an enzyme that in humans is encoded by the DHX9 gene. It is mapped to 1q25.3. This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

A0A8M3AQB4

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Dhx9 recombinant protein (amino acids Q194-K559) was used as the immunogen for the Zebrafish Dhx9 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, IF

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Dhx9 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Dhx9 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Dhx9 antibody should be determined by the researcher.

Image Legend

Immunohistochemical analysis of Dhx9 protein using Zebrafish Dhx9 antibody and paraffin-embedded zebrafish eye tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.