Zebrafish CrkL Antibody / Crk-like protein

Crk-like protein is a protein that in humans is encoded by the CRKL gene. This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

Q6PH06

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish CrkL recombinant protein (amino acids M1-H292) was used as the immunogen for the Zebrafish CrkL antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, IF

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish CrkL antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish CrkL antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish CrkL antibody should be determined by the researcher.

Location

Cytoplasmic, Nuclear

Image Legend

Immunofluorescent staining of FFPE zebrafish embryo tissue with Zebrafish CrkL antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.