Zebrafish Ddx6 Antibody / DEAD box protein 6

DDX6 (DEAD/H BOX 6), also known as HLR2 or p54, is an enzyme that in humans is encoded by the DDX6 gene. DDX6 belongs to the DEAD box family of putative RNA helicases that contain a characteristic asp-glu-ala-asp (DEAD) box motif (Seto et al., 1995) . Tunnacliffe et al. (1993) assigned the DDX6 gene more precisely using a panel of sequence tagged sites (STSs) representing 30 markers previously assigned to 11q23. Using mass spectroscopy, Fenger-Gron et al. (2005) found that RCK, EDC3 (YJDC), and HEDLS (RCD8) co-immunopurified with DCP1A and DCP2 from HEK293 cell lysates. Overexpression of DCP2, RCK, or EDC3 in HeLa cells reduced the association of endogenous DCP1A and XRN1 with cytoplasmic P bodies.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL

UniProt

E7FD91

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Ddx6 recombinant protein (amino acids I67-L484) was used as the immunogen for the Zebrafish Ddx6 antibody.

Clonality

Polyclonal

Isotype

Applications

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Ddx6 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Ddx6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Ddx6 antibody should be determined by the researcher.

Image Legend

Western blot analysis of Ddx6 protein using Zebrafish Ddx6 antibody and whole female zebrafish tissue lysate. The predicted molecular weight of Ddx6 is ~54 kDa.