Zebrafish Hypoxanthine phosphoribosyltransferase Antibody / Hprt1

Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is an enzyme encoded in humans by the HPRT1 gene. The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q7ZV49

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish HPRT1 recombinant protein (amino acids A29-R91) was used as the immunogen for the Zebrafish Hypoxanthine phosphoribosyltransferase antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Hypoxanthine phosphoribosyltransferase antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Hypoxanthine phosphoribosyltransferase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Hypoxanthine phosphoribosyltransferase antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE zebrafish brain tissue with Zebrafish Hypoxanthine phosphoribosyltransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.