Zebrafish Gbf1 Antibody / Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1

Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 is a protein that in humans is encoded by the GBF1 gene. This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

A0A286Y9X1

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Gbf1 recombinant protein (amino acids E110-K1605) was used as the immunogen for the Zebrafish Gbf1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, IF

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Gbf1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Gbf1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Gbf1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Immunofluorescent staining of FFPE zebrafish embryo tissue with Zebrafish Gbf1 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.