Zebrafish Nol10 Antibody / Nucleolar protein 10

By immunoprecipitation of human AATF it was discovered that AATF forms a salt-stable protein complex together with neuroguidin (NGDN) and NOL10 and demonstrated that the AATF-NGDN-NOL10 (ANN) complex functions in ribosome biogenesis. All three ANN complex members localize to nucleoli and display a mutual dependence with respect to protein stability. Mapping of protein-protein interaction domains revealed the importance of both the evolutionary conserved WD40 repeats in NOL10 and the UTP3/SAS10 domain in NGDN for complex formation.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q802W4

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Nol10 recombinant protein (amino acids Y73-D497) was used as the immunogen for the Zebrafish Nol10 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Nol10 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Nol10 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Nol10 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Immunohistochemical analysis of Nol10 protein using Zebrafish Nol10 antibody, HRP secondary and DAB substrate with paraffin-embedded zebrafish kidney tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.