Zebrafish Mettl3 Antibody / Mt-a70

N6-adenosine-methyltransferase 70 kDa subunit (METTL3) is an enzyme that in humans is encoded by theMETTL3 gene. It is mapped to 14q11.2. This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

F1R777

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Mettl3 recombinant protein (amino acids D381-D575) was used as the immunogen for the Zebrafish Mettl3 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Mettl3 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Mettl3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Mettl3 antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

IHC staining of Mettl3 protein using Zebrafish Mettl3 antibody, HRP secondary and DAB substrate with zebrafish colon tissue lysate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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