Zebrafish ATP binding cassette E1 Antibody / Abce1

ATP binding cassette E1 (ABCE1, also RNase L inhibitor) is an ATPase found in humans involved in viral assembly. It is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily. ABCE1 inhibits the action of ribonuclease L. Ribonuclease L normally binds to 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) and inhibits the interferon-regulated 2-5A/RNase L pathway, which is used by viruses. ABCE1 heterodimerize with ribonuclease L and prevents its interaction with 2-5A, antagonizing the anti-viral properties of ribonuclease L, and allow the virus to synthesize viral proteins. It has also been implicated to have an effect in tumorcell proliferation and antiapoptosis.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q6TNW3

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish ABCE1 recombinant protein (amino acids Q141-D599) was used as the immunogen for the Zebrafish ATP binding cassette E1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish ATP binding cassette E1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish ATP binding cassette E1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish ATP binding cassette E1 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE zebrafish brain tissue with Zebrafish ATP binding cassette E1 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.