Zebrafish Dnaja2 Antibody / Dnaja2a / Dnaja2b

DnaJ homolog subfamily A member 2 is a protein that in humans is encoded by the DNAJA2 gene. It is mapped to 16q11.2. The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F) -rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q7ZUP5

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish DNAJA2a/b recombinant protein (amino acids M1-Q230) was used as the immunogen for the Zebrafish Dnaja2 antibody. This antibody will detect the a and b isoforms.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Dnaja2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Dnaja2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish DNAJA2 antibody should be determined by the researcher.

Image Legend

Immunohistochemical analysis of DNAJA2a/b protein using DNAJA2 antibody and paraffin-embedded zebrafish pancreas tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.