Zebrafish Nampt Antibody / Nampt1 / Nicotinamide phosphoribosyltransferase

Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme that in humans is encoded by the PBEF1 gene. This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

E7F8T6

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Nampt1 recombinant protein (amino acids G62-N338) was used as the immunogen for the Zebrafish Nampt antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Nampt antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Nampt antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Nampt antibody should be determined by the researcher.

Location

Cytoplasm, nucleus, secreted

Image Legend

Immunohistochemical analysis of Nampt1 protein using Zebrafish Nampt antibody and paraffin-embedded zebrafish skeletal muscle tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.