Zebrafish Dlst Antibody / Dihydrolipoamide S-Succinyltransferase

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial is an enzyme that in humans is encoded by the DLST gene. This gene encodes a mitochondrial protein that belongs to the 2-oxoacid dehydrogenase family. This protein is one of the three components (the E2 component) of the 2-oxoglutarate dehydrogenase complex that catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO (2) . Alternatively spliced transcript variants have been found for this gene.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q7ZVL3

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Dlst recombinant protein (amino acids D86-M458) was used as the immunogen for the Zebrafish Dlst antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Dlst antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Dlst antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Dlst antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE zebrafish kidney tissue with Zebrafish Dlst antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.