Zebrafish Hmgb1 Antibody / Hmgb1a / Hmgb1b

High mobility group box 1 protein, also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a protein that in humans is encoded by the HMGB1 gene. This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 2-5 µg/mL, Western blot: 0.5-1 µg/mL

UniProt

Q6NX86, X1WFI9

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Hmgb1a/b recombinant protein (amino acids D123-K153) was used as the immunogen for the Zebrafish Hmgb1 antibody. This antibody will detect the a and b isoforms.

Clonality

Polyclonal

Isotype

Applications

IHC-P, WB

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish HMGB1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Hmgb1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Hmgb1 antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic, cell membrane, extracellular

Image Legend

Immunohistochemical analysis of Hmgb1a/b protein using Zebrafish Hmgb1 antibody, HRP secondary and DAB substrate with paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.