Zebrafish Ube2i Antibody / Ube2ia / Ube2ib

Enables SUMO transferase activity. Acts upstream of or within several processes, including embryonic heart tube development; hematopoietic stem cell homeostasis; and protein sumoylation. Predicted to be active in nucleus. Is expressed in several structures, including immature eye; nervous system; neural crest; pectoral fin bud; and pharyngeal pouch. Orthologous to human UBE2I (ubiquitin conjugating enzyme E2 I) .

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL

UniProt

Q9W6H5, Q9DDJ0

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Ube2ia/b recombinant protein (amino acids N124-S158) was used as the immunogen for the Zebrafish Ube2i antibody. This antibody will detect the a and b isoforms of Ube2i protein.

Clonality

Polyclonal

Isotype

Applications

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Ube2i antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Ube2i antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Ube2i antibody should be determined by the researcher.

Image Legend

Western blot analysis of Ube2ia/b protein using Zebrafish Ube2i antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Predicted molecular weight ~18 kDa.