Zebrafish Atg5 Antibody / Autophagy protein 5

Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is also known as APG5 or ASP, and this gene is mapped to 6q21. It is found that knockdown of ATG5 in hepatocytes increased triglyceride levels with oleate or a second endogenous stimulus for triglyceride formation. These hepatocytes with ATG5 knockdown also had increased lipid droplet number and size. ATG5 is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

F1RDC0

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Atg5 recombinant protein (amino acids S118-L155) was used as the immunogen for the Zebrafish Atg5 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Atg5 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Atg5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Atg5 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE zebrafish cerebellum tissue with Zebrafish Atg5 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.