Zebrafish Cct4 Antibody / TCP1 delta

T-complex protein 1 subunit delta is a protein that in humans is encoded by the CCT4 gene. This gene is mapped to 2p15. The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha-transducin.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

Q6PH46

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Cct4 recombinant protein (amino acids R446-R533) was used as the immunogen for the Zebrafish Cct4 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, IF

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Cct4 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Cct4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Cct4 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Immunofluorescent staining of FFPE zebrafish embryo tissue with Zebrafish Cct4 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.