Zebrafish Gnao1a Antibody / Guanine nucleotide-binding protein G (o) subunit alpha

Guanine nucleotide-binding protein G (o) subunit alpha is a protein that in humans is encoded by the GNAO1 gene. The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

Q6PBP1

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Gnao1a recombinant protein (amino acids C3-R179) was used as the immunogen for the Zebrafish Gnao1a antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, IF

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Gnao1a antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Gnao1a antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Gnao1a antibody should be determined by the researcher.

Location

Cytoplasm, cell membrane

Image Legend

Immunofluorescent staining of FFPE zebrafish embryo tissue with Zebrafish Gnao1a antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.