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Zebrafish Hmox1a Antibody / Hmox1 / Heme oxygenase

Hmox1a is inducible under a variety of stress conditions, including oxidative damage, hypoxia, heavy metal exposure, and inflammation. Its expression is regulated by transcription factors such as Nrf2 and Bach1. Zebrafish hmox1a is highly conserved and is expressed in multiple tissues including the liver, kidney, and vasculature, especially under stress or injury. Zebrafish provide a powerful in vivo model for studying Hmox1a function in developmental biology, toxicology, inflammation, and iron metabolism. Because of its conservation with mammalian HMOX1, hmox1a is widely used in research on antioxidant defense, redox signaling, and as a readout for environmental and pharmaceutical stress responses.

Product Specifications

Specifications

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

B0UXS0

Host

Rabbit

Reactivity

Zebrafish

Immunogen

E. coli-derived zebrafish Hmox1a recombinant protein (amino acids M1-K244) was used as the immunogen for the Zebrafish Hmox1a antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Hmox1a antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Hmox1a antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Hmox1a antibody should be determined by the researcher.

Location

Cytoplasm (ER)

Image Legend

IHC staining of FFPE zebrafish liver tissue with Zebrafish Hmox1a antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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