Zebrafish Hsd3b1 Antibody

Zebrafish hsd3b1 is expressed in steroidogenic tissues during development and in adult stages, where it supports endocrine functions related to stress response, reproduction, and growth. The enzyme is highly conserved across vertebrates, making zebrafish a valuable model for studying steroid biosynthesis, hormonal regulation, and the effects of endocrine-disrupting chemicals (EDCs) . Because of its involvement in hormone production and its responsiveness to physiological and environmental signals, zebrafish Hsd3b1 is widely used in research on developmental endocrinology, reproductive biology, and toxicology.

Product Specifications

Specifications

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

F1QSA2

Host

Rabbit

Reactivity

Zebrafish

Immunogen

E. coli-derived zebrafish Hsd3b1 recombinant protein (amino acids F16-K374) was used as the immunogen for the Zebrafish Hsd3b1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Hsd3b1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Hsd3b1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Hsd3b1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE zebrafish kidney tissue with Zebrafish Hsd3b1 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.