Zebrafish Fli1 Antibody / Friend leukemia integration 1 transcription factor

Friend leukemia integration 1 transcription factor (FLI1), also known as transcription factor ERGB, is a protein that in humans is encoded by the FLI1 gene, which is a proto-oncogene. It is mapped to 11q24.3. This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t (11;22) (q24; q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t (4;11) (q21; q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.

Product Specifications

Specifications

Western Blot: 0.5-1 µg/mL

UniProt

A0A8M9PLW9

Host

Rabbit

Reactivity

Zebrafish

Immunogen

E. coli-derived zebrafish Fli1 recombinant protein (amino acids M1-H426) was used as the immunogen for the Zebrafish Fli1 antibody.

Clonality

Polyclonal

Isotype

Applications

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Fli1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Fli1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Fli1 antibody should be determined by the researcher.

Image Legend

Western blot analysis of Fli1 protein using Zebrafish Fli1 antibody and 1) zebrafish head, 2) whole female zebrafish and 3) whole male zebrafish tissue lysate. Predicted molecular weight ~54 kDa.