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Zebrafish Hbae1.1 Antibody / Hemoglobin alpha embryonic 1.1

Hbae1.1 is expressed primarily in the intermediate cell mass, which is the site of primitive erythropoiesis in zebrafish embryos. It forms part of the embryonic hemoglobin complex that enables efficient oxygen delivery during rapid cell growth and organ formation. As development progresses, Hbae1.1 expression decreases while adult forms of hemoglobin become dominant. Due to its specific and transient expression pattern, Hbae1.1 is widely used as a molecular marker for primitive erythroid cells and early blood development in zebrafish. It provides a valuable readout for studying hematopoiesis, gene regulation during red blood cell formation, and the effects of genetic mutations or chemical exposures on early blood cell differentiation. Zebrafish Hbae1.1 is an important tool in developmental biology, hematology, toxicology, and genetic screening for blood related disorders.

Product Specifications

Specifications

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q7ZT21

Host

Rabbit

Reactivity

Zebrafish

Immunogen

E. coli-derived zebrafish Hbae1.1 recombinant protein (amino acids M1-R143) was used as the immunogen for the Zebrafish Hbae1.1 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Hbae1.1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Hbae1.1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Hbae1.1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE zebrafish heart tissue with Hbae1.1 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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