Zebrafish Polr2d Antibody / Rpb4 / DNA-directed RNA polymerase II subunit RPB4

DNA-directed RNA polymerase II subunit RPB4 is an enzyme that in humans is encoded by the POLR2D gene. This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

A0A8M1N062

Host

Rabbit

Reactivity

Zebrafish

Immunogen

E. coli-derived zebrafish Polr2d recombinant protein (amino acids A10-D127) was used as the immunogen for the Zebrafish Polr2d antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Polr2d antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Polr2d antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Polr2d antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of zebrafish Polr2d protein using Zebrafish Polr2d antibody, HRP-labeled secondary and DAB substrate. Polr2d was detected in a paraffin-embedded section of zebrafish spinal tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.