MyoD1 Antibody

Recognizes a phosphor-protein of 45kDa, identified as MyoD1. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.

Product Specifications

Specifications

Flow Cytometry: 0.5-1ug/million cells in 0.1ml, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

UniProt

P15172

Host

Mouse

Reactivity

Human, Mouse, Rat.

Immunogen

Recombinant mouse MyoD1 protein was used as the immunogen for this MyoD1 antibody. The epitope of this mAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

5.8A

Applications

FACS, IF, IHC

Purity

Protein G affinity chromatography

Format

Purified

Limitations

This MyoD1 antibody is available for research use only.

Storage Conditions

Store the MyoD1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.

Image Legend

IHC staining of FFPE human Rhabdomyosarcoma with MyoD1 antibody (clone 5.8A) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.