Anti-EpCAM Antibody / Extracellular domain

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P16422

Host

Mouse

Reactivity

Human, Dog, Cat

Immunogen

A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the anti-EpCAM antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

EGP40/1372

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Limitations

This anti-EpCAM antibody is available for research use only.

Storage Conditions

Store the anti-EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Applications Notes

Titering of the anti-EpCAM antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human hepatocellular carcinoma and anti-EpCAM antibody (clone EGP40/1372) . Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.