Dnmt3a Antibody

DNMT3A is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western Blot: 1-2 µg/mL

UniProt

Q16655

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full length human protein was used as the immunogen for this Dnmt3a antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PCRP-DNMT3A-1E2

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Limitations

This Dnmt3a antibody is available for research use only.

Storage Conditions

Store the Dnmt3a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Dnmt3a antibody to be titered up or down for optimal performance.

Location

Nuclear

Image Legend

IHC testing of FFPE human placenta with Dnmt3a antibody. Required HIER: boil sections in 1mM EDTA, pH 9, for 10-20 min and allow to cool before testing.