Dnmt3a Antibody
DNMT3A is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.
Product Specifications
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western Blot: 1-2 µg/mL
UniProt
Q16655
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full length human protein was used as the immunogen for this Dnmt3a antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
PCRP-DNMT3A-1E2
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Limitations
This Dnmt3a antibody is available for research use only.
Storage Conditions
Store the Dnmt3a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Nuclear
Image Legend
Frequently Asked Questions
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