PAX5 Antibody

The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding domain, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis; therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western bot: 2-4 µg/mL

UniProt

Q02548

Host

Mouse

Reactivity

Human

Immunogen

Full length human recombinant protein was used as the immunogen for this PAX5 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PCRP-PAX5-1B1

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Limitations

This PAX5 antibody is available for research use only.

Storage Conditions

Store the PAX5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PAX5 antibody to be titered up or down for optimal performance.