Cadherin 2 Antibody / CDH2 / N-Cadherin

Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL

UniProt

P19022

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 400-600 from human Cadherin 2 protein was used as the immunogen for the Cadherin 2 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CDH2/9489

Applications

IHC-P, WB

Purity

Protein G affinity

Format

Purified

Limitations

This Cadherin 2 antibody is available for research use only.

Storage Conditions

Aliquot the Cadherin 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Cadherin 2 antibody should be determined by the researcher.

Location

Cell surface, Cytoplasmic

Image Legend

IHC staining of FFPE human heart tissue with Cadherin 2 antibody (clone CDH2/9489) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.