Cadherin-2 Antibody / N-Cadherin / CDH2

Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P19022

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human Cadherin 2 partial protein from the cytoplasmic domain was used as the immunogen for the Cadherin 2 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CDH2/9491

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This Cadherin-2 antibody is available for research use only.

Storage Conditions

Aliquot the Cadherin 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Cadherin 2 antibody should be determined by the researcher.

Location

Cell Surface, Cytoplasm

Image Legend

IHC staining of FFPE human heart tissue with Cadherin 2 antibody (clone CDH2/9491) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.