Oligodendrocyte transcription factor 2 Antibody / OLIG2

Olig2, a basic helix-loop-helix transcription factor, is involved in oligo-dendroglial specification. Olig2 expression has been reported in most glial tumors, such as oligodendrogliomas and astrocytomas. Although more than half of glioblastomas are positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q13516

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-200 from human OLIG2 protein was used as the immunogen for the Oligodendrocyte transcription factor 2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

OLIG2/9995R

Applications

IHC-P

Purity

Protein A affinity

Format

Purified

Limitations

This Oligodendrocyte transcription factor 2 antibody is available for research use only.

Storage Conditions

Aliquot the Oligodendrocyte transcription factor 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Oligodendrocyte transcription factor 2 antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

IHC staining of FFPE human brain tissue with Oligodendrocyte transcription factor 2 antibody (clone OLIG2/9995R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.