Mesothelin Antibody / MSLN

Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Product Specifications

Specifications

Western blot: 1-2 µg/mL

UniProt

Q13421

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 273-407 from human Mesothelin protein was used as the immunogen for the Mesothelin antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

MSLN/2132

Applications

Purity

Protein G affinity

Format

Purified

Limitations

This Mesothelin antibody is available for research use only.

Storage Conditions

Aliquot the Mesothelin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Mesothelin antibody should be determined by the researcher.

Location

Cell membrane, Golgi apparatus, Secreted

Image Legend

Western blot testing of human OVCAR-3 cell lysate with Mesothelin antibody (clone MSLN/2132) . Predicted molecular weight ~69 kDa (precursor), ~40 kDa (cleaved form) . Glycosylation may make the protein run higher than expected.