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Recombinant MSLN Antibody / Mesothelin

Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL

UniProt

Q13421

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 250-450 from human Mesothelin protein was used as the immunogen for the recombinant MSLN antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

MSLN/8331R

Applications

IHC-P, WB

Purity

Protein A affinity

Format

Purified

Limitations

This recombinant MSLN antibody is available for research use only.

Storage Conditions

Aliquot the recombinant MSLN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant MSLN antibody should be determined by the researcher.

Location

Cell surface, Secreted

Image Legend

IHC staining of FFPE human ovarian carcinoma tissue with recombinant MSLN antibody (clone MSLN/8331R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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