Recombinant CDKN1B Antibody / p27Kip1
This MAb recognizes a 27kDa protein called p27Kip1/CDKN1B/Cyclin-dependent kinase inhibitor 1B, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb is excellent for staining of formalin-fixed tissues.
Product Specifications
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL
UniProt
P46527
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human CDKN1B protein was used as the immunogen for the recombinant CDKN1B antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG2a κ
Clone
RCDKN1B/13245
Applications
IHC-P, WB
Purity
Protein G affinity
Format
Purified
Limitations
This recombinant CDKN1B antibody is available for research use only.
Storage Conditions
Aliquot the recombinant CDKN1B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant CDKN1B antibody should be determined by the researcher.
Location
Cytoplasm, Endosome, Nucleus
Image Legend
Western blot testing of human SK-BR-3 cell lysate with recombinant CDKN1B antibody (clone rCDKN1B/13245) . Commonly observed at ~27 kDa.
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