CEA Antibody / CEACAM5

This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+) . Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

Product Specifications

Specifications

Western blot: 2-4 µg/mL, Immunohistochemistry: 1-2 µg/mL

UniProt

P06731

Host

Mouse

Reactivity

Human

Immunogen

Human CEA/CECAM5 protein was used as the immunogen for the CEA antibody.

Clonality

Monoclonal

Isotype

IgG κ

Clone

CEACAM5/12673

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Limitations

This CEA antibody is available for research use only.

Storage Conditions

Aliquot the CEA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CEA antibody should be determined by the researcher.

Location

Cytoplasm and luminal surface

Image Legend

Western blot testing of human colon tissue with CEA antibody (clone CEACAM5/12673) . Predicted molecular weight ~77 kDa but may be observed at higher molecular weights due to glycosylation.