Secretagogin Antibody / SCGN

SCGN, also known as Secretagogin, CALBL, setagin or SECRET, is a 276 amino acid cytoplasmic protein that contains six EF-hand domains and is related to the calicium-binding proteins Calretinin and Calbindin D28K. Expressed in a variety of tissues including stomach, thyroid, colon, brain and neuroendocrine cells, SCGN is thought to be involved in cell proliferation and KCl (potassium chloride) -mediated calcium flux events. Through its interaction with KCl and its subsequent ability to modulate calcium storage pools within the cell, SCGN may function to negatively control growth and differentiation rates and, thus, indirectly inhibit cell replication.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

O76038

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 100-276 from human SCGN protein was used as the immunogen for the Secretagogin antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

SCGN/7325

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This Secretagogin antibody is available for research use only.

Storage Conditions

Aliquot the Secretagogin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Secretagogin antibody should be determined by the researcher.

Location

Cytoplasm, Secreted

Image Legend

IHC staining of FFPE human pancreas tissue with Secretagogin antibody (clone SCGN/7325) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.