Cofilin 2 Antibody / CFL2

Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous (F) Actin by bridging two longitudinally associated Actin subunits changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser-3 by factors such as opsonized zymosan. Lim kinase 1, a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerise F-Actin.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q9Y281

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-166 from human CFL2 protein was used as the immunogen for the Cofilin 2 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CFL2/9963

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This Cofilin 2 antibody is available for research use only.

Storage Conditions

Aliquot the Cofilin 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Cofilin 2 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human skeletal muscle tissue with Cofilin 2 antibody (clone CFL2/9963) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.