Clusterin Antibody / Apolipoprotein J / APO-J

Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, ä¼ª and å°¾, are associated through disulfide bonds. The å°¾ subunit (also called ApoJä¼ª) corresponds to residues 23-227. The ä¼ª subunit (also called ApoJå°¾) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences å°¾-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P10909

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 150-300 from human CLU protein was used as the immunogen for the Clusterin antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CLU/4736

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This Clusterin antibody is available for research use only.

Storage Conditions

Aliquot the Clusterin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Clusterin antibody should be determined by the researcher.

Location

Cytoplasm, Secreted

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using Clusterin antibody (clone CLU/4736) . These results demonstrate the foremost specificity of the CLU/4736 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.