CPS1 Antibody / Carbamoyl phosphate synthetase 1

This MAb recognizes a protein of 165kDa, identified as carbamoyl phosphate synthetase 1 (CPS1) . This mitochondrial enzyme catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

P31327

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 800-1000 from human CPS1 protein was used as the immunogen for the CPS1 antibody.

Clonality

Monoclonal

Isotype

IgG κ

Clone

CPS1/9859

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Limitations

This CPS1 antibody is available for research use only.

Storage Conditions

Aliquot the CPS1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CPS1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human hepatocellular carcinoma tissue with CPS1 antibody (clone CPS1/9859) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.