CYP2C9 Antibody / Cytochrome P450 2C9

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P11712

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-200 from the human protein was used as the immunogen for the CYP2C9 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CYP2C9/13121

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This CYP2C9 antibody is available for research use only.

Storage Conditions

Aliquot the CYP2C9 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CYP2C9 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human hepatocellular carcinoma tissue with CYP2C9 antibody (clone CYP2C9/13121) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.