Dihydrodiol dehydrogenase 1 Antibody / DDH1 / AKR1C1

DDH1 / AKR1C1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

Q04828

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human full-length AKR1C1 protein was used as the immunogen for the Dihydrodiol dehydrogenase 1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AKR1C1/9318

Applications

IHC-P, WB

Purity

Protein G affinity

Format

Purified

Limitations

This Dihydrodiol dehydrogenase 1 antibody is available for research use only.

Storage Conditions

Aliquot the Dihydrodiol dehydrogenase 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Dihydrodiol dehydrogenase 1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human hepatocellular carcinoma tissue with Dihydrodiol dehydrogenase 1 antibody (clone AKR1C1/9318) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.