Recombinant HER2 Antibody / ErbB2 / Extracellular region

Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P04626

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein from human ERBB2 corresponding to the extracellular domain (ECD) was used as the immunogen for the recombinant HER-2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

ERBB2/13055R

Applications

IHC-P

Format

Purified

Limitations

This recombinant HER-2 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant HER-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant HER-2 antibody should be determined by the researcher.

Location

Cell membrane

Image Legend

IHC staining of FFPE human breast cancer tissue with recombinant HER-2 antibody (clone ERBB2/13055R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.