CD135 Antibody / FLT3

Stem cell tyrosine kinase (STK-1) has been cloned from a CD34+ hematopoietic stem cell enriched library and identified as the human homolog of a previously identified gene of Mouse origin designated either Flk-2 or Flt-3. The STK-1 cDNA encodes a protein of 993 amino acids with 85% identity to Flt-3/Flk-2. STK-1 is a member of the type III receptor tyrosine kinase family that includes Kit (steel factor receptor), Fms and PDGF. STK-1 expression in blood and marrow is restricted to CD34+ cells, a population greatly enriched for hematopoietic stem/progenitor cells. STK-1 antiserum recognizes two polypeptides in these cells. The Mouse homolog of STK-1, designated Flt-3/Flk-2, is expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues. It has been suggested that STK-1 and its murine homolog Flt-3/Flk-2 may function as growth factor receptors on hematopoietic stem and/or progenitor cells.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P36888

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 500-800 from human FLT3 protein was used as the immunogen for the CD135 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

FLT3/9888

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This CD135 antibody is available for research use only.

Storage Conditions

Aliquot the CD135 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CD135 antibody should be determined by the researcher.

Location

Endoplasmic reticulum lumen, Membrane

Image Legend

IHC staining of FFPE human brain tissue with CD135 antibody (clone FLT3/9888) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.