GFAP Antibody / Glial Fibrillary Acidic Protein

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP) . It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system.Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

P14136

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 150-350 from human GFAP protein was used as the immunogen for the GFAP antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

GFAP/9422

Applications

IHC-P, WB

Purity

Protein G affinity

Format

Purified

Limitations

This GFAP antibody is available for research use only.

Storage Conditions

Aliquot the GFAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the GFAP antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/9422) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.