GFAP Antibody / Glial Fibrillary Acidic Protein
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP) . It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system.Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Product Specifications
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL
UniProt
P14136
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 100-400 from human GFAP protein was used as the immunogen for the GFAP antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
GFAP/9423
Applications
IHC-P, WB
Purity
Protein G affinity
Format
Purified
Limitations
This GFAP antibody is available for research use only.
Storage Conditions
Aliquot the GFAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the GFAP antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human brain tissue with GFAP antibody (clone GFAP/9423) . Predicted molecular weight ~50 kDa.
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