GFAP Antibody / Glial Fibrillary Acidic Protein

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

P14136

Host

Mouse

Reactivity

Human

Immunogen

A recombinant full-length human GFAP protein was used as the immunogen for the GFAP antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

GFAP/9424

Applications

IHC-P, WB

Purity

Protein G affinity

Format

Purified

Limitations

This GFAP antibody is available for research use only.

Storage Conditions

Aliquot the GFAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the GFAP antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/9424) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.